NRAS - the most frequent pathogenic variants_somatic mutation analysis

NRAS: codons 12, 13, 59, 61, 117 and 146

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GENE / VARIANT / LOCUS

NRAS: codons 12, 13, 59, 61, 117 and 146

SPECIALIZATIONS

Oncology

METHODOLOGY

Qualitative real-time PCR (IDYLA),Qualitative real-time PCR (IDYLA),Real-time PCR,Real-time PCR

TAT (DAYS)

10 calendar days

OMIM CODE (GENE/LOCUS)

164790

OMIM CODE (FENOTYPE)

114500

RefSNP ID

ACCREDITATION

Yes

SAMPLE REQUIREMENTS

Sample type	Volume/Concentration¹	Container	Transport temperature	Sample viability (days/hours)²
DNA extracted from tissue	≥ 50 µl [50 ng/µl]	Eppendorf Safe-lock sterile tube	Room temp.	n/a
DNA extracted from tissue	≥ 50 µl [50 ng/µl]	Eppendorf Safe-lock sterile tube	Room temp.	n/a
Peripheral blood	≥ 10 ml [2x]	Cell-Free DNA BCT	Room temp.	1 (ASAP)
Peripheral blood	≥ 10 ml [2x]	Cell-Free DNA BCT	Room temp.	1 (ASAP)
Tissue	Block	processed with buffered formalin	Room temp.	n/a
Tissue	2 FFPE tissue cuts [5 – 10 µm] with ≥ 10 % tumor cells	Eppendorf Safe-lock sterile tube	Room temp.	2
Tissue	Block	processed with buffered formalin	Room temp.	n/a
Tissue	2 FFPE tissue cuts [5 – 10 µm] with ≥ 10 % tumor cells	Eppendorf Safe-lock sterile tube	Room temp.	2

¹ If it is not possible to collect the minimum volume for the test, please contact Unilabs Genetics

² Maximum shipping time to ensure sample quality